Effect of ethanol extracts of Antrodia cinnamomea on head and neck squamous cell carcinoma cell line

Figures

Figure 1. Ethanol extracts of Antrodia cinnamomea…

Figure 1. Ethanol extracts of Antrodia cinnamomea (EEA) inhibited cell proliferation in a dose-dependent manner.…

Figure 1. Ethanol extracts of Antrodia cinnamomea (EEA) inhibited cell proliferation in a dose-dependent manner. A, SAS cells were treated with EEA ranging from 0.5 to 25 μg/m, and proliferation inhibition was significantly observed in cells treated with EEA over 2.5 μg/mL after incubation for 5 days. B, Cleaved caspase-9 and cleaved PARP were increased in cells treated with EEA. Data are reported as means±SD. *P<0.05, **P<0.01, ***P< 0.001 (ANOVA).

Figure 2. Suppression of wounding healing by…

Figure 2. Suppression of wounding healing by ethanol extracts of Antrodia cinnamomea (EEA). A ,…

Figure 2. Suppression of wounding healing by ethanol extracts of Antrodia cinnamomea (EEA). A, A wound was made when cell confluence was 100% and fresh medium supplemented with different concentrations of EEA was added. Images were captured at 0 and 36 h. B, Migration distance was calculated and normalized to control that was not treated with EEA. Data are reported as means±SD. **P<0.01, ***P<0.001 compared to control (ANOVA).

Figure 3. Inhibitive effects of ethanol extracts…

Figure 3. Inhibitive effects of ethanol extracts of Antrodia cinnamomea (EEA) on SAS cell invasion.…

Figure 3. Inhibitive effects of ethanol extracts of Antrodia cinnamomea (EEA) on SAS cell invasion. A, SAS cells were plated into the upper chamber in medium supplemented with various concentrations of EEA. After 36-h incubation, invading cells were counted in five randomly selected fields (scale bars: 50 μm. B, The average counts of invading cells were significantly less in groups treated with EEA than in control. C, Effects of EEA on invasion-associated proteins, including MMP-2, MMP-9, TIMP-1, and TIMP-2, were evaluated by western blot. Data are reported as means±SD. **P<0.01 (ANOVA).

Figure 4. Inhibitive effects of ethanol extracts…

Figure 4. Inhibitive effects of ethanol extracts of Antrodia cinnamomea (EEA) on tumorigenesis in vivo…

Figure 4. Inhibitive effects of ethanol extracts of Antrodia cinnamomea (EEA) on tumorigenesis in vivo. A and B, Nude BALB/c mice were inoculated with SAS cells and treated with EEA when tumors were palpable. Tumor growth was significantly inhibited by EEA administration. Data are reported as means±SD. ***P<0.001 (ANOVA).