Chemical quality evaluation of Antrodia cinnamomea fruiting bodies using phytomics similarity index analysis
Abstract
The fruiting body of Antrodia cinnamomea is used as a medicinal mushroom in Taiwan and is found on the inner cavity of the endemic species Cinnamomum kanehirai. In this study, phytomics similarity index (PSI) analysis was employed for the chemical quality evaluation of the A. cinnamomea fruiting bodies from different strains, and grown on various substrates. The results indicated that the different types of A. cinnamomea fruiting bodies contain eight index compounds, and that it was difficult to discriminate between them solely on the basis of those index compounds. In our research, we used PSI scores to assess the metabolite similarity of the fruiting bodies of A. cinnamomea. It was revealed that fruiting bodies from various A. cinnamomea strains grown on different culture substrates produce distinct PSI scores. We concluded that PSI analysis had good selectivity on the different types of A. cinnamomea fruiting bodies.
Figures 
Fig. 1
Morphological observations of Antrodia cinnamomea…
Fig. 1
Morphological observations of Antrodia cinnamomea fruiting bodies in this study.
Fig. 1 Morphological observations of Antrodia cinnamomea fruiting bodies in this study.
Fig. 2
HPLC chemical fingerprint of ethanol…
Fig. 2
HPLC chemical fingerprint of ethanol extract of A. cinnamomea fruiting body strain BCRC…
Fig. 2 HPLC chemical fingerprint of ethanol extract of A. cinnamomea fruiting body strain BCRC 35398. The index compounds are A: (R, S)-Antcin K. B: 1,4-Dimethoxy-2,3-methylenedioxy-5-methylbenzene. C:(R,S) -Antcin C. D:(R,S)-Antcin H. E: Dehydrosulfurenic acid. F: (R,S)-Antcin B. G: (R,S)-Antcin A. H: Dehydroeburicoic acid.
Fig. 3
HPLC chemical fingerprint of ethanol…
Fig. 3
HPLC chemical fingerprint of ethanol extract of fruiting bodies of different A. cinnamomea…
Fig. 3 HPLC chemical fingerprint of ethanol extract of fruiting bodies of different A. cinnamomea strains. The index compounds are A: (R, S)-Antcin K. B: 1,4-Dimethoxy-2,3-methylenedioxy-5-methylbenzene. C:(R,S)-Antcin C. D:(R,S)-Antcin H. E: Dehydrosulfurenic acid. F: (R,S)-Antcin B. G: (R,S)-Antcin A. H: Dehydroeburicoic acid.
Fig. 4
HPLC chemical fingerprint of ethanol…
Fig. 4
HPLC chemical fingerprint of ethanol extract of A. cinnamomea fruiting bodies grown on…
Fig. 4 HPLC chemical fingerprint of ethanol extract of A. cinnamomea fruiting bodies grown on Cinnamomum kanehirai wood (35398), Cinnamomum camphora (CC) wood, Cunninghamia konishii wood (CK), Cunninghamia lanceolata (CL) wood, and dish culture (DC). The index compounds are A: (R, S)-Antcin K. B: 1,4-Dimethoxy-2,3-methylenedioxy-5-methylbenzene. C:(R,S)-Antcin C. D:(R,S)-Antcin H. E: Dehydrosulfurenic acid. F: (R,S)-Antcin B. G: (R,S)-Antcin A. H: Dehydroeburicoic acid.