Medicinal Fungus Antrodia cinnamomea Inhibits Growth and Cancer Stem Cell Characteristics of Hepatocellular Carcinoma
Figures
Figure 1
Antitumor effect of AC -MFB…
Figure 1
Antitumor effect of AC -MFB on BALB/c liver cancer. Effect of AC -MFB…
Figure 1 Antitumor effect of AC-MFB on BALB/c liver cancer. Effect of AC-MFB on growth of 1MEA.7R.1 liver cancer cells in BALB/c mice. Mice were treated with oral AC-MFB (50 mg/mL) or normal saline for 28 days. *P < 0.05.
Figure 2
Cytotoxicity of AC -MFB on…
Figure 2
Cytotoxicity of AC -MFB on hepatocellular carcinoma cell. Effect of AC -MFB on…
Figure 2 Cytotoxicity of AC-MFB on hepatocellular carcinoma cell. Effect of AC-MFB on cellular viability of HA22T and 1MEA.7R.1 HCC cells by MTT assay. Data from three separate experiments were expressed as percentages of untreated cells after 24 and 48 h.
Figure 3
Cytotoxicity of AC -MFB on…
Figure 3
Cytotoxicity of AC -MFB on endothelial cell. Effect of AC -MFB on cellular…
Figure 3 Cytotoxicity of AC-MFB on endothelial cell. Effect of AC-MFB on cellular viability of EA. hy926 and SVEC4-10 ECs by MTT assay. Data from three separate experiments were expressed as percentages of untreated cells after 24 and 48 h.
Figure 4
Migration inhibition of AC -MFB…
Figure 4
Migration inhibition of AC -MFB on endothelial cell. Effect of AC -MFB on…
Figure 4 Migration inhibition of AC-MFB on endothelial cell. Effect of AC-MFB on migration of EA. hy926 ((a)–(d)) and SVEC4-10 ((e)–(h)) ECs by in vitro wound-healing migration assay. Data from three separate experiments were expressed as mean inhibition percentages compared with untreated cells after 24 h treatment.
Figure 5
Tube formation inhibition of AC…
Figure 5
Tube formation inhibition of AC -MFB on endothelial cell. Effect of AC -MFB…
Figure 5 Tube formation inhibition of AC-MFB on endothelial cell. Effect of AC-MFB on tube formation activity of EA. hy926 ((a)–(d)) and SVEC4-10 ((e)–(h)) ECs. Data from three separate experiments were expressed as mean inhibition percentages compared with untreated cells after 12 h treatment.
Figure 6
AC -MFB inhibits VEGF from…Figure 6
AC -MFB inhibits VEGF from hepatocellular cancer cell. Effect of various concentrations of… Figure 6 AC-MFB inhibits VEGF from hepatocellular cancer cell. Effect of various concentrations of AC-MFB on extracellular VEGF levels in culture of HA22T and 1MEA.7R.1 HCC cells. Cells were pretreated with 200 μM DFO for 2 h followed by AC-MFB for 6 h. All the data are normalized to the control group (AC-MFB 0.0 mg/mL). Data from three independent experiments in triplicate were expressed as mean ± standard error of mean; *P < 0.05 versus 200 μM DFO.
Figure 7
AC -MFB inhibits HIF-1 α…Figure 7
AC -MFB inhibits HIF-1 α from hepatocellular cancer cell. Effect of various concentrations… Figure 7 AC-MFB inhibits HIF-1α from hepatocellular cancer cell. Effect of various concentrations of AC-MFB on intracellular HIF-1α levels in culture of HA22T and 1MEA.7R.1 HCC cells. Cells were pretreated with 200 μM DFO for 2 h followed by AC-MFB for 2 h. All the data were normalized to the control group (AC-MFB 0 mg/mL). Data from three independent experiments in triplicate were expressed as mean ± standard error of mean; *P < 0.05 versus 200 μM DFO. All figures (7)